TY -非盟的邹一轩盟,邵Juanjuan AU -李,释永信盟——赵F.-Q。Jian-Xin盟盟- Liu -刘,据说PY - 2019 DA - 2019/03/26 TI -保护无机和有机硒对热应力的影响在牛乳腺上皮细胞SP - 1503478六世- 2019 AB -当奶牛暴露在高温环境中,他们的抗氧化能力和生产性能下降,导致经济损失。证据表明硒(Se)可以有效地缓解热应激奶牛;然而,细胞保护机制还不清楚。本研究的目的是调查和比较无机硒的保护作用(亚硒酸钠,SS)和有机硒(亚硒酸蛋氨酸,SM) MAC-T(乳腺肺泡细胞大小的T抗原,牛乳腺上皮细胞(BMEC)线)细胞在热应力。MAC-T细胞治疗在4方面另有描述:(i)热处理(HT)组织细胞培养为42.5°C 1 h,然后恢复37°C 12 h;(2)SM组使用有机Se 2 h,培养为42.5°C 1 h,然后恢复37°C 12 h;(3)SS组治疗类似于SM组除了细胞使用无机Se代替有机硒;及(iv)对照组在37°C和不断培养没有收到Se治疗。结果表明,热休克为42.5°C 1 h引发热休克反应,破坏了氧化还原平衡,并减少在MAC-T细胞细胞生存能力; and pretreatment of cells with SM or SS effectively alleviated the negative effects of heat shock on the cells. However, the cells were much more sensitive to SS treatment but more tolerant to SM. In addition, two forms of Se appeared to affect the expression of different genes, including nuclear factor erythroid 2-related factor 2 (Nrf2) and inducible nitric oxide synthase (iNOS) in the SM group and thioredoxin reductase 1 (TXNRD1) in the SS group in Nrf2-ARE (antioxidant response element) antioxidant pathway and inflammation response. In summary, results showed the mechanistic differences in the protective effects of organic and inorganic Se on heat stress in BMECs. SN - 1942-0900 UR - https://doi.org/10.1155/2019/1503478 DO - 10.1155/2019/1503478 JF - Oxidative Medicine and Cellular Longevity PB - Hindawi KW - ER -