TY - Jour A2 - Giustarina，Daniela Au - Carnevale，Roberto Au - Biondi-Zoccai，Giuseppe Au - Peruzzi，Mariangela Au - de Falco，Elena Au - Chimenti，Isotta Au - Venuta，Federico Au - Anile，Marco Au - Diso，Daniele AU - Cavarretta, Elena AU - Marullo, Antonino G. M. AU - Sartini, Patrizio AU - Pignatelli, Pasquale AU - Violi, Francesco AU - Frati, Giacomo PY - 2014 DA - 2014/04/16 TI - New Insights into the Steen Solution Properties: Breakthrough in Antioxidant Effects via NOX2 Downregulation SP - 242180 VL - 2014 AB - Ex vivo lung perfusion (EVLP) allows perfusion and reconditioning of retrieved lungs for organ transplantation. The Steen solution is specifically designed for this procedure but the mechanism through which it elicits its activity is still to be fully clarified. We speculated that Steen solution may encompass antioxidant properties allowing a reestablishment of pulmonary tissue homeostasis. Blood samples from 10 healthy volunteers were recruited. Platelets and white cells were incubated with Steen solution or buffer solution as control and stimulated with suitable agonists. Reactive oxidant species (ROS), soluble NOX2 (sNOX2-derived peptide), a marker of NADPH oxidase activation, P. 47. Phox. 在细胞刺激时测量以氧化应激的标记，作为氧化应激的标志物，作为氧化应激的标志物，强大的血管扩张剂和抗氧化分子的易位。Steen溶液显着抑制 P. 47. Phox. 血小板和白色细胞中的易位和NOx2激活。与该发现一致的是通过血小板和白色细胞的显着降低的ROS和异丙醇的形成，减少氧化应激。最后，与尺寸溶液的细胞孵育导致增强的NO产生。在此，我们提供了第一种证据，即Steen溶液通过NADPH氧化酶活性的下调具有抗氧化性能，并增强了NO的产生。SN - 1942-0900 UR - https://doi.org/10.1155/2014/242180 Do - 10.1155/2014 / 242180 JF - 氧化医学和蜂窝长寿PB - Hindwai Publishing CorporationKW - ER -