TY - JOUR A2 - Minghetti, Luisa AU - Crispo, James A. G. AU - Ansell, Dominique R. AU - Ubriaco, Gino AU - Tai, T. C. PY - 2011 DA - 2011/10/09 TI - Role of Reactive Oxygen Species in the Neural and Hormonal Regulation of the PNMT Gene in PC12 Cells SP - 756938 VL - 2011 AB - The stress hormone, epinephrine, is produced predominantly by adrenal chromaffin cells and its biosynthesis is regulated by the enzyme phenylethanolamine N-methyltransferase (PNMT). Studies have demonstrated that PNMT may be regulated hormonally via the hypothalamic-pituitary-adrenal axis and neurally via the stimulation of the splanchnic nerve. Additionally, hypoxia has been shown to play a key role in the regulation of PNMT. The purpose of this study was to examine the impact of reactive oxygen species (ROS) produced by the hypoxia mimetic agent CoCl₂上PNMT的在体外细胞培养物模型中的激素和神经的刺激，利用大鼠嗜铬细胞瘤（PC12）细胞系。RT-PCR分析的PNMT显示导入内含子的保持和内含子的mRNA的剪接通过氯化钴变体₂（3.0和1.76倍，分别地）。细胞的瞬时转染测定用氯化钴同时处理₂和合成糖皮质激素，地塞米松，显示出增加的启动子活性（18.5倍），同时兼具剪接变体的mRNA水平没有表现出协同效应。调查氯化钴的效果时，观察到类似的结果₂在PNMT的通过毛喉素神经刺激诱导的ROS。我们的研究结果表明，氯化钴₂诱导ROS对PNMT启动子的激素和神经激活的协同效应。SN - 1942-0900 UR - https://doi.org/10.1155/2011/756938 DO - 10.1155 /七十五万六千九百三十八分之二千○一十一JF - 氧化医学和细胞寿命PB - Hindawi出版公司公司KW - ER -