TY - A2的阿里•谢赫Md赛义德盟方,Xiangming盟歌,仁生AU -魏,嘉兴盟——廖,秦盟——曾Zhenhong PY - 2022 DA - 2022/09/27 TI -挖掘潜在的药物靶点和心脏衰竭的诊断模型构建基于miRNA-mRNA网络SP - 9652169六世- 2022 AB -心力衰竭(HF)是一个全球流行的心血管疾病,但仍然缺乏有效的药物靶点和诊断模型。本研究旨在探讨有效的高频药物靶点和诊断模型的microrna的目标,希望能有助于理解和治疗心力衰竭。使用高频microrna从地理数据库和基因表达谱数据,我们分析了差异表达microrna /基因识别高频使用Limma在线TargetScan数据库和预测microrna的目标。随后,基因集富集分析和注释进行使用WebGestaltR包。蛋白质相互作用数据库被确定使用字符串。近距离治疗心力衰竭的药物也计算并预测潜在目标的治疗药物。此外,进一步的药物识别是由分子对接。最后,诊断模型建立了基于微分microrna。地理数据集被用来屏幕66 microrna的异常表达,包含56个表达下调microrna和10调节microrna。JAK-STAT信号通路,MAPK信号通路,p53信号通路,催乳激素信号通路,及信号通路和丰富,见KEGG浓缩分析目标基因。 In addition, we found that 83 genes were upregulated and 92 genes were downregulated in HF patients vs. healthy individuals. Based on the inflammation-related score, hypoxia-related score, and energy metabolism-related score, we identified key miRNA-mRNA pairs and constructed an interaction network. Following that, TAP1, which had the highest expression and network connectivity in acute HF with crystal and molecular docking studies, was selected as a key candidate gene in the network. And the compound DB04847 was selected to produce a large number of favorable interactions with TAP1 protein. Finally, we constructed two diagnostic models based on the differential miRNAs hsa-miR-6785-5p and hsa-miR-4443. In conclusion, we identified TAP1, a key candidate gene in the diagnosis and treatment of HF, and determined that compound DB04847 is highly likely to be a potential inhibitor of TAP1. The TAP1 gene was also found to be regulated by hsa-miR-6785-5p and hsa-miR-4443, and a diagnostic model was constructed. This provides a new promising direction to improve the diagnosis, prognosis, and treatment outcome and guide more effective immunotherapy strategies of HF. SN - 0962-9351 UR - https://doi.org/10.1155/2022/9652169 DO - 10.1155/2022/9652169 JF - Mediators of Inflammation PB - Hindawi KW - ER -