TY - JOUR A2 - Andrukhov，奥莱AU - Funel，Niccola AU - 迪尼，瓦伦蒂娜AU - Janowska，阿加塔AU - Loggini，芭芭拉AU - Minale，的Massimiliano AU - GRIECO，Fabrizia AU - 里奇奥，萨尔瓦多AU - 罗马内利，马尔科PY -- 2851949 VL - 2020 AB - 基质金属蛋白酶的生物活性炎性途径与分子机制伤口愈合SP：2020 DA - - 2020年2月27日TI在BV-2细胞小麦大麦提取物调节蛋白激酶B和基质金属蛋白酶9蛋白表达的蛋白酶（MMPs）是一个大家族具有蛋白水解活性遍在表达的锌依赖性酶。它们在涉及炎性过程，包括上皮至间质转变（EMT），神经元损伤和癌症生理状况和病理状况中表达。还有证据表明，蛋白酶在肿瘤微环境，它在愈合过程中的组织的重要作用调节炎症。寻找在两个炎症和神经元损害，MMP9参与在这两个过程及其调制似乎是由两种蛋白来调节：肿瘤坏死因子-α（TNF-α）和白细胞介素6（IL-6）。然而，其他重要的基因参与转录因子，蛋白激酶B（AKT），和p65分子调控。此外，小麦大麦提取物（TVE）调制与炎性过程，包括p65蛋白相关联的生物标志物。虽然没有证据表明TVE可能在其它炎症标记物作为AKT的生物调制参与，我们想评估TVE是否能够AKT（pAKT的）的作为体外炎症过程的早期标志物（1）调制的磷酸化和（2）影响在体外模型MMP9蛋白的表达。 The BV-2 cells (microglial of mouse) have been used as an in vitro model to simulate both inflammatory and neuronal injury pathologies. Here, MMP9 seems to be involved in cellular migration through inflammatory marker activation. We simulate an inflammatory preclinical model treating BV-2 cells with lipopolysaccharide (LPS) to induce proinflammatory activation affecting pAKT and p65 proteins. TVE is revealed to restore the native expression of AKT and p65. Additionally, TVE extract modulates also the protein concentration of MMP9. Nevertheless, immunofluorescence confocal analyses revealed that both AKT and MMP9 are regulated together, synchronously. This work seems to demonstrate that two important genes can be used to monitor the beginning of an inflammatory process, AKT and MMP9, in which TVE seems able to modulate their expression of inflammation-associated molecules. SN - 0962-9351 UR - https://doi.org/10.1155/2020/2851949 DO - 10.1155/2020/2851949 JF - Mediators of Inflammation PB - Hindawi KW - ER -