TY -的A2 Caccamo Daniela AU - Wang Yun-peng盟——郭,于非盟-温,Ping-shan盟——赵甄蛰AU -谢,剑盟-杨,凯盟-杨,清王盟——,Jia-feng AU -邓,小明PY - 2020 DA - 2020/02/29 TI -三成分红花减轻急性肺损伤和抑制脂多糖引起的净释放SP - 2720369六世- 2020 AB - Xuebijing注入是一种中药化合物治疗脓毒症在中国,但它包含了许多不同的组件,并且每种成分在治疗脓毒症时可能有不同的效果。本实验研究血碧净三种成分:红花黄A (SYA)、羟基红花黄A (HSYA)和anhydrosafflor yellow B (AHSYB)对脂多糖- (LPS-)诱导的急性肺损伤(ALI)的影响。气管内注射LPS (10 mg/kg)致小鼠急性肺损伤，用SYA、HSYA和AHSYB治疗。收集血液、支气管肺泡灌洗液(BALF)和肺组织，以检测肺损伤程度、炎症水平和中性粒细胞胞外陷阱(NETs)。采用肉豆蔻醋酸酯(phorbol myristate acetate, PMA)刺激HL-60细胞进行体外实验。组织病理学检查显示，SYA、HSYA和AHSYB均能减轻LPS所致的肺损伤。这三种成分可以抑制脂多糖诱导的炎症因子水平、干湿比以及BALF中蛋白质和细胞数量的升高。在免疫荧光检测中，它们还显著减少了髓过氧化物酶(MPO)和组蛋白的覆盖，降低了血浆中MPO- dna复合物的水平。体外实验显示，三种成分均抑制了中性粒细胞样HL-60细胞中pma诱导的净释放。 Western blot demonstrated that phosphorylation of c-rapidly accelerated fibrosarcoma (c-Raf), mitogen-activated protein kinase ERK kinase (MEK), and extracellular signal-regulated kinase (ERK) in the lungs of LPS-challenged mice, and PMA-treated HL-60 cells were all significantly reduced by SYA, HSYA, and AHSYB. Therefore, our data demonstrated that three components of XBJ, including SYA, HSYA, and AHSYB, showed a protective effect against LPS-induced lung injury and NET release. SN - 0962-9351 UR - https://doi.org/10.1155/2020/2720369 DO - 10.1155/2020/2720369 JF - Mediators of Inflammation PB - Hindawi KW - ER -