TY -的A2他Shaoheng AU - Luz,里卡多·阿尔维斯AU - Xavier-Elsas,佩德罗AU - de Luca比安卡盟——Masid-de-Brito Daniela盟——Cauduro巴西Soares AU -贡德尔Arcanjo,路易斯卡洛斯AU - Cordeiro法多斯桑托斯,安娜卡非盟- de Oliveira新克里斯蒂娜•玛丽亚盟——Gaspar-Elsas玛丽亚Ignez五车二PY - 2014 DA - 2014/02/25 TI - 5-Lipoxygenase-Dependent招聘Eotaxin-Stimulated小鼠中性粒细胞和巨噬细胞的嗜酸性粒细胞SP - 102160六世- 2014 AB -嗜酸性粒细胞在抗菌防御的角色仍不完全清楚。在ovalbumin-sensitized老鼠,嗜酸性粒细胞被抗原选择性地招募到腹腔,eotaxin,或白三烯B4 (LT),一个5-lipoxygenase 5-LO代谢物。阻止反应抗原和eotaxin 5-LO封锁。我们检查了应对eotaxin没有5-LO敏感和他们的依赖。BALB / c或不是老鼠及其突变体(5-LO-deficient ALOX;与eotaxin eosinophil-deficient GATA-1)注入ip,嗜酸性粒细胞,或两者兼而有之,白细胞积累量化24 h。显著的嗜酸性粒细胞的招聘eotaxin BALB / c, 24 h,伴随着大量招募中性粒细胞和单核细胞/巨噬细胞。这些影响被eotaxin中和废除,5-LO-activating蛋白质抑制剂MK886。在ALOX(但不是PAS)小鼠,eotaxin招聘为嗜酸性粒细胞,中性粒细胞减少一半被废除。在GATA-1突变体,eotaxin招募中性粒细胞和巨噬细胞。 Transfer of eosinophils cultured from bone-marrow of BALB/c donors, or from ALOX donors, into GATA-1 mutant recipients, i.p., restored eotaxin recruitment of neutrophils and showed that the critical step dependent on 5-LO is the initial recruitment of eosinophils by eotaxin, not the secondary neutrophil accumulation. Eosinophil-dependent recruitment of neutrophils in naive BALB/c mice was associated with increased binding of bacteria. SN - 0962-9351 UR - https://doi.org/10.1155/2014/102160 DO - 10.1155/2014/102160 JF - Mediators of Inflammation PB - Hindawi Publishing Corporation KW - ER -