TY -的A2 Raczyńska这样非盟- Puddu,亚历山德拉AU - Sanguineti,罗伯塔非盟- Montecucco,法盟——Viviani,乔治•l . PY - 2013 DA - 2013/11/06 TI -视网膜色素上皮细胞表达功能受体Glucagon-Like Peptide-1六世(GLP-1) SP - 975032 - 2013 AB - Glucagon-Like Peptide-1 (GLP-1)是一个gut-derived肠促胰岛素激素,已经被证明可以改善2型糖尿病的葡萄糖稳态。GLP-1的生物效应是由其特定的受体GLP-1R表达在一个广泛的组织,在那里负责的extra-pancreatic GLP-1的影响。由于视网膜色素上皮(RPE),形成外层视网膜屏障,有一个关键的角色在保护糖尿病性视网膜病变(DR),我们调查了潜在的表达和功能的GLP-1R RPE细胞。ARPE-19细胞培养在DMEM / F12补充10%的边后卫。GLP-1R是评估在mRNA的表达和蛋白质的水平。然后,激活postreceptor胞内信号转导途径(细胞外signal-regulated激酶1和2 (ERK1/2)和蛋白激酶B (PKB))被评估免疫印迹在正常细胞或沉默的GLP-1R 10 nmol / L GLP-1的存在与否。潜在的胞内信号通路之间的连接由GLP-1刺激进行孵化前细胞增殖蛋白激酶的激酶药理抑制剂(MEK) 1/2, phosphatydilinositol-3kinase (PI3K)和表皮生长因子受体(EGFR)。结果表明,GLP1R ARPE-19 mRNA和蛋白表达水平的细胞。刺激和GLP-1强烈激活PKB ERK1/2磷酸化直到40分钟的曝光。GLP-1-mediated激酶的活化依赖于上游PI3K和表皮生长因子受体的激活。 Finally, treatment with GLP-1 did not affect the spontaneous release of VEGF-A from ARPE-19 cells. In conclusion, this paper showed that the presence of functional GLP-1R is expressed in RPE cells. These data might represent the rationale to further investigate the potential direct beneficial effects of GLP-1 treatment against DR. SN - 0962-9351 UR - https://doi.org/10.1155/2013/975032 DO - 10.1155/2013/975032 JF - Mediators of Inflammation PB - Hindawi Publishing Corporation KW - ER -