TY -的A2 -摩纳哥,克劳迪娅盟——张,明非盟- Wang Chun-Mei AU - Li Jing AU -孟,赵杰AU -魏,剩男非盟-李,霁AU -布克拉,理查德·李盟- yu lin AU -陈,李PY - 2013 DA - 2013/12/09 TI -小檗碱防止Palmitate-Induced内皮功能障碍:参与AMPK和eNOS上调和NOX4 SP - 260464 VL - 2013 AB -内皮功能障碍是糖尿病心血管并发症发生的关键因素。小檗碱可改善糖尿病引起的内皮功能障碍。然而，潜在的机制仍不清楚。本研究旨在探讨小檗碱对棕榈酸盐诱导的人脐静脉内皮细胞(HUVECs)内皮功能障碍的保护作用及其机制。MTT法测定HUVECs细胞活力。测定上清液或培养的HUVECs中一氧化氮(NO)水平和活性氧(ROS)的生成。RT-PCR检测内皮型一氧化氮合酶(eNOS) mRNA水平，分析eNOS、p-eNOS、Akt、p-Akt、AMPK、p-AMPK、NADPH氧化酶(NOX4)蛋白水平。结果表明，小檗碱能显著提高NO水平，减少ROS的产生。在小檗碱处理的HUVECs中，eNOS表达显著增加，NOX4蛋白表达降低。 Moreover, berberine upregulated the protein expression of AMPK and p-AMPK in palmitate-treated HUVECs, but had no effect on the levels of Akt. Therefore, berberine ameliorates palmitate-induced endothelial dysfunction by upregulating eNOS expression and downregulating expression of NOX4. This regulatory effect of berberine may be related to the activation of AMPK. SN - 0962-9351 UR - https://doi.org/10.1155/2013/260464 DO - 10.1155/2013/260464 JF - Mediators of Inflammation PB - Hindawi Publishing Corporation KW - ER -