TY -的A2 -钟,魏盟- Ju,燕盟——元,本盟,吴Wenan盟——赵,金盟——史,Xiaorui PY - 2021 DA - 2021/12/28 TI - CircRNA ANXA2促进肺癌细胞增殖和转移的移植PDPK1表达式SP - 4526609六世- 2021 AB -肺癌是一种常见的恶性肿瘤,严重威胁着人类健康。它已成为恶性肿瘤的发病率和死亡率。近年来,circRNA,一种特殊的非编码RNA分子,已经吸引了相当大的兴趣。本研究关注的角色circRNA ANXA2 (circANXA2)在肺癌和促进癌症的分子机制。实时定量聚合酶链反应(rt - PCR)是用于检测circANXA2的表达丰度在不同肺癌细胞和组织。的亚细胞定位circANXA2通过荧光原位杂交检测。通过核circANXA2表达式被撞倒了。CCK-8、克隆形成试验和TUNEL分析被用于评估circANXA2对细胞增殖的影响,克隆形成能力,和细胞凋亡。circANXA2在肿瘤增殖的作用进一步验证在裸小鼠体内使用肿瘤移植模型。circANXA2的分子机制是研究荧光素酶活性测定和rt - pcr。 The expression abundance of circANXA2 is high in lung cancer cell lines and tissues. Knocking down of circANXA2 inhibits the proliferation and clonogenesis of the lung cancer cells. Knocking down circANXA2 promotes apoptosis. circANXA2 further affects downstream PDPK1 expression by regulating miR-33a-5p and thereby affecting the malignancy of the lung cancer cells. circANXA2 inhibits miR-33a-5p activity by directly interacting with miR-33a-5p. circANXA2 regulates the transcription of the miR-33a-5p downstream target gene PDPK1 and affects the malignant progression of lung cancer. SN - 1687-8450 UR - https://doi.org/10.1155/2021/4526609 DO - 10.1155/2021/4526609 JF - Journal of Oncology PB - Hindawi KW - ER -