TY -的A2 -李,Chia-Jung AU - Souza,凯伦c . b . AU -伊万格丽斯塔,Adriane f . AU - Leal, Leticia f . AU - Souza, c p . AU -维埃拉,雷内·a . AU - Causin Rhafaela l . AU - Neuber a . c . AU -萨姆,丹尼尔·p . AU - Passos Geraldo a . s . AU -里斯,鲁伊·m . v . AU -品牌,Marcia M. C. PY - 2019 DA - 2019/08/08 TI - Identification of Cell-Free Circulating microrna for Detection of Early Breast Cancer and Molecular Subtyping SP - 8393769 VL - 2019 AB -早期检测对降低乳腺癌死亡率至关重要。肿瘤患者血清中循环细胞游离microrna的分析已经成为一种有前景的新的非侵袭性生物标志物，用于肿瘤的早期检测和预测其分子分类。这项研究的基本原理是确定亚型特异性的细胞游离microrna的分子谱，用于早期检测血清中的乳腺癌。选取54例早期乳腺癌患者，27例年龄匹配对照进行血清中循环microrna的评估。54例患者进行分子分型(luminal A、luminal B、luminal B Her2阳性、Her-2阳性、三阴性)。利用NanoString平台对800个标记的microRNA探针进行数字化检测和定量，并比较乳腺癌患者与对照组血清microRNA表达的总体差异。我们在每个分子亚型中筛选出42个差异最显著(P≤0.05,1.5倍)的循环microrna进行进一步研究。在这些microrna中，19个在管腔A患者中显著表达，8个在管腔B患者中表达，10个在管腔B HER2阳性患者中表达，4个在HER2富集亚型患者中表达。AUC高，敏感性和特异性合适。 For the triple negative subtype miR-25-3p had the best accuracy. Predictive analysis of the mRNA targets suggests they encode proteins involved in molecular pathways such as cell adhesion, migration, and proliferation. This study identified subtype-specific molecular profiles of cell-free microRNAs suitable for early detection of breast cancer selected by comparison to the microRNA profile in serum for female controls without apparent risk of breast cancer. This molecular profile should be validated using larger cohort studies to confirm the potential of these miRNA for future use as early detection biomarkers that could avoid unnecessary biopsy in patients with a suspicion of breast cancer. SN - 1687-8450 UR - https://doi.org/10.1155/2019/8393769 DO - 10.1155/2019/8393769 JF - Journal of Oncology PB - Hindawi KW - ER -