TY - JOUR A2 - Eritja，拉蒙AU - 摩根，蕾哈娜K. AU - 莫尔纳，迈克尔M. AU - 巴特拉，Harshul AU - 萨摩福特，伯大尼AU - Wadkins，兰迪M. AU - 布鲁克斯，麦蒂A. PY - 2018 DA- 2018年5月16日TI - 5-羟甲基表观遗传修饰对VEGF的稳定性和分子识别的影响的i-Motif和G-四链结构SP - 9281286 VL - 2018 AB - 启动子常常包含不对称G-和C-富链，其中所述胞嘧啶是通过甲基（5-MC）和5-羟甲基化（5-HMC）易发生表观遗传修饰。这些序列还可以形成四股G-四（G4）或I基序（IM）的二级结构。尽管后生调制和IM / G4形成所需的序列是相似的并且可以重叠，它们不可能并存。尽管5-hmC的为5-MC的氧化产物，这两个修饰的碱基在群集不同位点。This study focuses on the intersection of G4/iM formation and 5-hmC modification using the vascular endothelial growth factor (VEGF) gene promoter’s CpG sites and examines whether incorporation of 5-hmC into iM/G4 structures had any physicochemical effect on formation, stability, or recognition by nucleolin or the cationic porphyrin, TMPyP4. No marked changes were found in the formation or stability of iM and G4 structures; however, changes in recognition by nucleolin or TMPyP4 occurred with 5-hmC modification wherein protein and compound binding to 5-hmC modified G4s was notably reduced. G4/iM structures in the VEGF promoter are promising therapeutic targets for antiangiogenic therapy, and this work contributes to a comprehensive understanding of their governing principles related to potential transcriptional control and targeting. SN - 2090-0201 UR - https://doi.org/10.1155/2018/9281286 DO - 10.1155/2018/9281286 JF - Journal of Nucleic Acids PB - Hindawi KW - ER -