TY - JOUR A2 - Llorent-Martínez, Eulogio J. AU - Gan, Ning AU - Jia, Liyong AU - Zheng, Lei PY - 2011 DA - 2011/10/13 TI - A Novel Sandwich Electrochemical Immunosensor Based on the DNA-Derived Magnetic Nanochain Probes for Alpha-Fetoprotein SP - 957805 VL - 2011 AB - One novel electrochemical immunosensor was constructed by immobilizing capture antibody of alpha-fetoprotein (AFP Ab₁) on a nafion/nanogold-particle modified glassy carbon electrode. With a sandwich immunoassay, one DNA-derived magnetic nanoprobe, simplified as DNA/(ZMPs—HRP-AFP Ab₂)_n, was employed for the detection of AFP. The fabricated procedure of the proposed biosensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The performance and factors influencing the performance of the biosensor were also evaluated. Under optimal conditions, the developed biosensor exhibited a well-defined electrochemicalbehavior toward the reduction of AFP ranging from 0.01 to 200 ng/mL with a detection limit of 4 pg/mL ( S / N = 3 ). The biosensor was applied to the determination of AFP in serum with satisfactory results. It is important to note that the sandwich nanochainmodified electro-immunosensor provided an alternative substrate for the immobilization of other tumor markers. SN - 2090-8865 UR - https://doi.org/10.1155/2011/957805 DO - 10.1155/2011/957805 JF - Journal of Automated Methods and Management in Chemistry PB - Hindawi Publishing Corporation KW - ER -