TY - Jour A2 - Bach，Leon Au - Ma，Fengxia Au - Chen，方奥 - 志，莹奥 - 阳，绍兴奥鲁，石宏奥 - 韩，忠剑，张超PY - 2012年DA - 2012/12/20 TI -用造血干细胞表面标志物分离胰腺祖细胞SP - 948683 VL - 2012 AB - 用造血干细胞表面标志物分离胰腺祖细胞，干细胞抗原（SCA-1）和C-kit的表达在小鼠胚胎胰腺中，检查它们用胰腺十二指肠Homeobox-1（PDX-1），神经原素3（NGN3）和胰岛素的共表达。然后通过磁体活化细胞分选分离不同的胰腺细胞亚群。在悬挂液滴中培养分离的细胞过夜。当细胞形成球体时，它们被铺设在空气/介质界面处的浮动过滤器上。通过这种新的培养系统，诱导胰腺祖细胞分化为内分泌和外泌体细胞。结果表明，12.5,15.5和妊娠17.5天的胚胎胰腺中的C-kit和SCA-1不同。C-kit和SCA-1的表达是妊娠15.5天的最高。C-kit而不是SCA-1与PDX-1，NGN3和胰岛素共表达。 Cells differentiated from c-Kit-positive cells contained more insulin-producing cells and secreted more insulin in response to glucose stimulation than that from c-Kit-negative cells. These results suggested that c-Kit could be used to isolate pancreatic progenitor cells and our new culture system permitted pancreatic progenitor cells to differentiate to mature endocrine cells. SN - 1687-8337 UR - https://doi.org/10.1155/2012/948683 DO - 10.1155/2012/948683 JF - International Journal of Endocrinology PB - Hindawi Publishing Corporation KW - ER -