TY - JOUR A2 - El-Baz, Ayman AU - Muñoz-Barrutia, Arrate AU - Ceresa, Mario AU - Artaechevarria, Xabier AU - Montuenga, Luis M. AU - Ortiz-de-Solorzano, Carlos PY - 2012 DA - 2012/11/08 TI - Quantification of Lung Damage in an Elastase-Induced Mouse Model of Emphysema SP - 734734 VL - 2012 AB - Objective. To define the sensitivity of microcomputed tomography- (micro-CT-) derived descriptors for the quantification of lung damage caused by elastase instillation. Materials and Methods. The lungs of 30 elastase treated and 30 control A/J mice were analyzed 1, 6, 12, and 24 hours and 7 and 17 days after elastase instillation using (i) breath-hold-gated micro-CT, (ii) pulmonary function tests (PFTs), (iii) RT-PCR for RNA cytokine expression, and (iv) histomorphometry. For the latter, an automatic, parallel software toolset was implemented that computes the airspace enlargement descriptors: mean linear intercept ( L m ) 一个nd weighted means of airspace diameters ( D 0 , D 1 , and D 2 ). A Support Vector Classifier was trained and tested based on three nonhistological descriptors using D 2 一个s ground truth. Results. D 2 发现统计上显著差异 ( P < 0.01 ) between the groups at all time points. Furthermore, D 2 一个t 1 hour (24 hours) was significantly lower ( P < 0.01 ) than D 2 一个t 24 hours (7 days). The classifier trained on the micro-CT-derived descriptors achieves an area under the curve (AUC) of 0.95 well above the others (PFTS AUC = 0.71; cytokine AUC = 0.88). Conclusion. Micro-CT-derived descriptors are more sensitive than the other methods compared, to detect in vivoearly signs of the disease. SN - 1687-4188 UR - https://doi.org/10.1155/2012/734734 DO - 10.1155/2012/734734 JF - International Journal of Biomedical Imaging PB - Hindawi Publishing Corporation KW - ER -