TY - JOUR A2 - Uchiyama, Kazuhiko AU - Qiao, Huixia AU - Huang, Yahui AU - Chen, Xiaoyan AU - Yang, Long AU - Wang, Yue AU - Xu, Rongrong PY - 2020 DA - 2020/05/27 TI - Jiaweishaoyao Decoction Alleviates DSS-Induced Ulcerative Colitis via Inhibiting Inflammation SP - 7182874 VL - 2020 AB - Purpose. Jiaweishaoyao decoction (JWSYD) is a traditional prescription of Chinese medicine that is initially used for the treatment of diarrhea. This study is aimed at investigating the effects of JWSYD on DSS-induced ulcerative colitis (UC). Methods. DSS-induced UC mice and LPS-induced RAW264.7 cells were used as the UC model in vivoand in vitro. UC was assessed by body weight, disease activity index (DAI), colon length, spleen weight, and histopathological score (HE staining). The levels of TNF- α,il - 1 β, and IL-6 were analyzed by ELISA and qRT-PCR. The levels of NLRP3 inflammasome- and NF- κB pathway-associated proteins were measured by western blot. Results. JWSYD alleviated DSS-induced UC in respect to body weight, DAI, colon length, spleen weight, and histopathological score. JWSYD reduced the levels of TNF- α,il - 1 β, and IL-6 in DSS-induced UC mice and the supernatants of LPS-induced RAW264.7 cells. JWSYD suppressed the protein levels of inflammasome-associated proteins, including NLRP3, ASC1, Procaspase-1, Cleaved caspase-1, and Cleaved IL-1 βin DSS-induced UC mice and LPS-induced RAW264.7 cells. In addition, JWSYD suppressed the NF- κB pathway in vitroand in vivo. Conclusion. JWSYD alleviated DSS-induced UC via inhibiting the NLRP3 inflammasome and NF- κB通路。SN - 1687 - 6121你的——https://doi。org/10.1155/2020/7182874 DO - 10.1155/2020/7182874 JF - Gastroenterology Research and Practice PB - Hindawi KW - ER -