TY - JOUR A2 - Santos, Renato L. AU - Carreira, Vinicius Soares AU - Ferrari, Heitor Flávio AU - Langohr, Ingeborg Maria AU - Mackenzie, Charles AU - Montezzo, Luiz Carlos AU - Taira, Edson AU - Floeter-Winter, Lucile Maria AU - Luvizotto, Maria Cecília Rui PY - 2014 DA - 2014/08/24 TI -Leishmaniasp. Amastigotes Identification in Canine Transmissible Venereal Tumor SP - 603852 VL - 2014 AB - Leishmaniasis is a vector-borne disease with Leishmania chagasibeing the etiological agent of canine visceral leishmaniasis in South America. Canine venereal tumor is a transplantable round cell tumor of histiocytic origin which is mostly observed in sexually active male and female intact dogs. It has been shown that Leishmaniaamastigotes have higher tropism for the canine male genital tract tissues and venereal leishmaniasis transmission has been documented in dogs but, to date, a canine venereal tumor-dependent transmission route has not been fully demonstrated. In this report, a 10-year-old, mixed breed, intact female dog presented a vaginal venereal transmissible tumor but no other clinical abnormalities otherwise. Unexpectedly, tumor tissue imprint smears examination revealed Leishmaniasp. amastigotes within infiltrating macrophages. In addition to the cytological direct identification, the protozoan was confirmed within the neoplastic tissue by means of immunohistochemistry and polymerase chain reaction. This report illustrates an asymptomatic Leishmaniasp. infection that may have started on or from the canine venereal tumor tissue, the latter option further supporting previous evidence of such an alternative vector-independent route of transmission for canine visceral leishmaniasis in areas where these diseases coexist. SN - 2090-7001 UR - https://doi.org/10.1155/2014/603852 DO - 10.1155/2014/603852 JF - Case Reports in Veterinary Medicine PB - Hindawi Publishing Corporation KW - ER -