Osamah TY -的A2思•易卜拉欣盟——熊齐盛盟——王濛盟——刘Jinglong AU -林,Chia-Ying PY - 2021 DA - 2021/12/13 TI -乳腺癌细胞转移到组织工程Premetastatic利基使用Osteoid-Formed聚已酸内酯/ Nanohydroxyapatite脚手架SP - 9354202六世- 2021 AB -这被认为是Premetastatic利基(中性粒细胞)中扮演着一个关键的角色在促进骨转移的乳腺癌细胞。组织工程支架提供一个有利的环境,促进骨生成可能模仿骨premetastatic利基市场(bpmn)。在这项研究中,人类间充质干细胞(hMSCs)被播种到设计聚已酸内酯/ nanohydroxyapatite (PCL-nHA)支架成骨分化。随后,coculture系统用于建立培养乳腺癌细胞的组织工程bpmn, hMSCs, osteoid-formed PCL-nHA支架。之后,迁移试验被用来调查mda - mb - 231的招聘,MCF-7, mda - mb - 453细胞bpmn的上层清液。癌症干细胞(CSC)这些迁移细胞的性质进行了流式细胞术。我们的研究结果表明,碱性磷酸酶(ALP)的mRNA表达水平,Osterix, runt-related转录因子2 (Runx2)和α1型胶原(COL1A1) PCL-nHA支架相比,大大增加了PCL支架在第11天,18岁和32岁。CXCL12的表达在这些bpmn在coculturing逐渐增加时间,它可能是一个可行的bpmn的标志。此外,迁移分析结果显示,高成熟的bpmn共同导致了外建立一个更有利的癌症入侵网站。乳腺癌干细胞的族群(BCSCs)更有可能迁移到肥沃的bpmn。 The proportion of BCSCs in metastatic MDA-MB-231, MCF-7, and MDA-MB-453 cells were increased by approximately 63.47%, 149.48%, and 127.60%. The current study demonstrated that a designed tissue engineering scaffold can provide a novel method to create a bone-mimicking environment that serves as a useable platform to recapitulate the BPMNs and help interrogate the scheme of bone metastasis by breast cancer. SN - 1748-670X UR - https://doi.org/10.1155/2021/9354202 DO - 10.1155/2021/9354202 JF - Computational and Mathematical Methods in Medicine PB - Hindawi KW - ER -