TY -的A2 -黄,道盟——高,掸族盟——朱城东街盟——朱,剑盟——沈Lianqiang盟——朱,明非盟- Ren,雪峰PY - 2021 DA - 2021/06/15 TI - miR-18a-5p目标FBP1促进增殖、迁移和入侵肝癌细胞和抑制细胞凋亡SP - 3334065六世- 2021 AB -肝癌是最激进的恶性肿瘤之一。重要的是理解肝癌细胞的分子机制开发新的治疗方案。研究发现,FBP1作为肿瘤抑制基因。FBP1的作用机理研究肝癌细胞,生物信息学分析来研究其在肝癌组织中表达。生存分析也执行。此外,母星数据库应用于预测FBP1的上游调控基因。Dual-luciferase试验进行作证他们的目标的关系。FBP1 mRNA和蛋白表达水平的肝癌细胞检测到存在和免疫印迹,分别。细胞生存能力被CCK-8化验分析。细胞的迁徙和侵入性能力被Transwell化验分析。 The apoptosis of liver cancer cells was detected by flow cytometry. The results showed that the expression of FBP1 was downregulated in liver cancer tissue and cells. FBP1 low expression was correlated with the poor prognosis of patients. miR-18a-5p could inhibit FBP1 expression. Overexpression of FBP1 could inhibit the progression of liver cancer cells and promote cell apoptosis. Overexpressing miR-18a-5p could promote the progression of liver cancer cells and inhibit cell apoptosis. However, overexpressing FBP1 simultaneously could reverse the effect. miR-18a-5p and FBP1 are expected to be candidates for liver cancer treatment. SN - 1748-670X UR - https://doi.org/10.1155/2021/3334065 DO - 10.1155/2021/3334065 JF - Computational and Mathematical Methods in Medicine PB - Hindawi KW - ER -