TY - JOUR A2 - 石，彭军AU - 科埃略，迭戈F. AU - 萨图尼诺，泰国庇隆AU - 费尔南德斯，费尔南达弗雷塔斯AU - 马佐拉，Priscila加瓦AU - 西尔韦拉，埃德加AU - Tambourgi，埃利亚斯巴西莱PY - 2016 DA - 2016/01/27 TI - Azocasein Substrate for Determination of Proteolytic Activity: Reexamining a Traditional Method Using Bromelain Samples SP - 8409183 VL - 2016 AB - Given the importance of protease’s worldwide market, the determination of optimum conditions and the development of a standard protocol are critical during selection of a reliable method to determine its bioactivity. This paper uses quality control theory to validate a modified version of a method proposed by Charney and Tomarelli in 1947. The results obtained showed that using azocasein substrate bromelain had its optimum at 45°C and pH 9 (Glycine-NaOH 100 mM). We also quantified the limit of detection (LoD) and limit of quantification (LoQ) in the above-mentioned optimum (0.072 and 0.494 mg·mL^-1的偶氮酪蛋白，RESP）和一个校准曲线相关因素与底物的量的光密度消化。在所有分析的样品中，我们在存储（约17％），这表明它的使用必须制备后立即响应后观察到显著降低。因此，该协议在本文报价提出了显著改善，考虑到主观的定义在文献中普遍使用这种简单的数学方法使得简洁明了。SN - 2314-6133 UR - https://doi.org/10.1155/2016/8409183 DO - 10.1155 /八百四十○万九千一百八十三分之二千零十六JF - 生物医学研究国际PB - Hindawi出版公司公司KW - ER -