ty -jour a2 -ciccarella，giuseppe au -aalami，阿米尔·霍斯辛（Amir Hossein au） - 梅斯加里（Mesgari），穆罕默德（Mohammad au），穆罕默德·苏（Mohammad au -Sahebkar），阿米尔霍斯（Amirhossein py）-2020 da -2020/11/20/20 ti-与绿色氧化物nanoparticle的合成和特征，通过抗皮质的合成和表征，and MicroRNA Modulation in Breast Cancer Cells SP - 8817110 VL - 2020 AB - Changes in the expression of microRNAs can affect cancer cells’ viability and behavior and the impact on cancer treatment. In this study, the expression of miR-155-5p, miR-203a-3p, and miR-223-3p in the MCF7 cancer cell line was studied when exposed to ZnO nanoparticles synthesized through a green route. Mentioned ZnO-NPs were well characterized by UV-vis spectroscopy, DLS, XRD, FTIR, FE-SEM, EDX, zeta potential, and AFM analyses. Cellular studies were conducted using ZnO-NPs before miRNA investigations including MTT cytotoxicity test against MCF7, MDA-MB-231, and HFF cell lines. Moreover, apoptosis assays were performed using morphological analysis, fluorescent dyes, flow cytometry, and evaluation of caspase-3 and caspase-8 gene expression. Biological properties such as the antioxidant and antimicrobial activity of these novel ZnO-NPs were considered. MTT assays showed that the inhibitory concentration (IC₅₀24小时后的ZnO-NPS为11.16  μg/ml，60.08  μg/ml和26.3  μMCF7，MDA-MB-231和HFF细胞上的G/mL。QRT-PCR结果显示，当MCF7细胞用IC50浓度的ZnO-NPs处理时，miR-155-5p，miR-203a-3p和miR-223-3p的表达降低（11.16） μg/ml）。抗氧化活性结果显示EC₅₀值为57.19  μg/ml和31.5  μDPPH和ABTS测定中的g/ml。使用MIC和MBC分析确定ZnO-NP的抗菌活性在革兰氏阴性和革兰氏阴性细菌菌株和真菌上确定。这些NP在降低乳腺癌细胞中microRNA的表达方面具有显着影响。最后，ZnO-NP施加了抗氧化剂和抗菌活性。SN -1565-3633 UR -https：//doi.org/10.1155/2020/8817110 do -10.1155/2020/8817110 JF-生物促进化学和应用PB -Hindawi kw -er -er-- er- er- er-