TY -的A2 Verma Subhash c . AU - Chan k h . AU - k . k . w . AU -李,p . t . w . AU -黄,t . l . AU -张,r . AU -小鸡,k . k . h . AU - Chan g . AU - Yip, c . c . y . AU -陈,h·l . AU -挂,i f . n . AU - Chan j·f·w . AU -袁,刘贤PY - 2017 DA - 2017/08/29 TI -评价NxTAG呼吸道病原体面板和与xTAG呼吸道病毒面板快速v2和电影数组呼吸面板检测鼻咽吸入呼吸道病原体和猪/ Avian-Origin甲型流感亚型在文化隔离SP - 1324276六世- 2017 AB -本研究评估a new multiplex kit, Luminex NxTAG Respiratory Pathogen Panel, for respiratory pathogens and compared it with xTAG RVP Fast v2 and FilmArray Respiratory Panel using nasopharyngeal aspirate specimens and culture isolates of different swine/avian-origin influenza A subtypes (H2N2, H5N1, H7N9, H5N6, and H9N2). NxTAG RPP gave sensitivity of 95.2%, specificity of 99.6%, PPV of 93.5%, and NPV of 99.7%. NxTAG RPP, xTAG RVP, and FilmArray RP had highly concordant performance among each other for the detection of respiratory pathogens. The mean analytic sensitivity (TCID50/ml) of NxTAG RPP, xTAG RVP, and FilmArray RP for detection of swine/avian-origin influenza A subtype isolates was 0.7, 41.8, and 0.8, respectively. All three multiplex assays correctly typed and genotyped the influenza viruses, except for NxTAG RRP that could not distinguish H3N2 from H3N2v. Further investigation should be performed if H3N2v is suspected to be the cause of disease. Sensitive and specific laboratory diagnosis of all influenza A viruses subtypes is especially essential in certain epidemic regions, such as Southeast Asia. The results of this study should help clinical laboratory professionals to be aware of the different performances of commercially available molecular multiplex RT-PCR assays that are commonly adopted in many clinical diagnostic laboratories. SN - 1687-8639 UR - https://doi.org/10.1155/2017/1324276 DO - 10.1155/2017/1324276 JF - Advances in Virology PB - Hindawi KW - ER -