TY - JOUR A2 - Hussain, Ghulam AU - Jamdade, Dhiraj A. AU - Rajpali, Dishantsingh AU - Joshi, Komal A. AU - Kitture, Rohini AU - Kulkarni, Anuja S. AU - Shinde, Vaishali S. AU - Bellare, Jayesh AU - Babiya, Kaushik R. AU - Ghosh, Sougata PY - 2019 DA - 2019/02/11 TI -Gnidia glauca- andPlumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents SP - 9080279 VL - 2019 AB - Rapid, eco-friendly, and cost-effective one-pot synthesis of copper nanoparticles is reported here using medicinal plants like Gnidia glaucaand Plumbago zeylanica. Aqueous extracts of flower, leaf, and stem of G. glaucaand leaves of P. zeylanicawere prepared which could effectively reduce Cu²⁺ions to CuNPs within 5 h at 100°C which were further characterized using UV-visible spectroscopy, field emission scanning electron microscopy, high-resolution transmission electron microscopy, energy dispersive spectroscopy, dynamic light scattering, X-ray diffraction, and Fourier-transform infrared spectroscopy. Further, the CuNPs were checked for antidiabetic activity using porcine pancreatic α-amylase and α-glucosidase inhibition followed by evaluation of mechanism using circular dichroism spectroscopy. CuNPs were found to be predominantly spherical in nature with a diameter ranging from 1 to 5 nm. The phenolics and flavonoids in the extracts might play a critical role in the synthesis and stabilization process. Significant change in the peak at ∼1095 cm⁻¹corresponding to C-O-C bond in ether was observed. CuNPs could inhibit porcine pancreatic α-amylase up to 30% to 50%, while they exhibited a more significant inhibition of α-glucosidase from 70% to 88%. The mechanism of enzyme inhibition was attributed due to the conformational change owing to drastic alteration of secondary structure by CuNPs. This is the first study of its kind that provides a strong scientific rationale that phytogenic CuNPs synthesized using G. glaucaand P. zeylanica可以考虑开发候选人antidiabeti吗c nanomedicine. SN - 2633-4682 UR - https://doi.org/10.1155/2019/9080279 DO - 10.1155/2019/9080279 JF - Advances in Pharmacological Sciences PB - Hindawi KW - ER -