TY - JOUR A2 - Schmitt, Fernando AU - Milano, Annalisa AU - Mazzetta, Francesca AU - Valente, Sabatino AU - Ranieri, Danilo AU - Leone, Laura AU - Botticelli, Andrea AU - Onesti, Concetta Elisa AU - Lauro, Salvatore AU - Raffa, Salvatore AU - Torrisi, Maria Rosaria AU - Marchetti, Paolo PY - 2018 DA - 2018/02/27 TI - Molecular Detection of EMT Markers in Circulating Tumor Cells from Metastatic Non-Small Cell Lung Cancer Patients: Potential Role in Clinical Practice SP - 3506874 VL - 2018 AB - Background. Non-small cell lung cancer (NSCLC) is the most common cause of cancer-related mortality; nevertheless, there are few data regarding detection of circulating tumor cells (CTCs) in NSCLC, compared to other kinds of cancers in which their prognostic roles have already been defined. This difference is likely due to detection methods based on the epithelial marker expression which ignore CTCs undergoing epithelial-mesenchymal transition (CTCs^EMT). Methods. After optimization of the test with spiking experiments of A549 cells undergoing TGF- β1-induced EMT (A549^EMT), the CTCs^EMTwere enriched by immunomagnetic depletion of leukocytes and then characterized by a RT-PCR assay based on the retrieval of epithelial and EMT-related genes. Blood samples from ten metastatic NSCLC patients before starting treatment and during chemotherapy were used to test this approach by longitudinal monitoring. Ten age- and sex-matched healthy subjects were also enrolled as controls. Results. Recovery experiments of spiked A549^EMTcells showed that the RT-PCR assay is a reliable method for detection of CTCs^EMT. CTCs^EMTwere detected in three patients at baseline and in six patients after four cycles of cysplatin-based chemotherapy. Longitudinal monitoring of three patients showed that the CTCs^EMTdetection is related to poor therapeutic response. Conclusions. The RT-PCR-based approach for the evaluation of CTCs^EMT表型可能是一种很有前途的和便宜的l to predict the prognosis and the therapeutic response in NSCLC patients. SN - 2210-7177 UR - https://doi.org/10.1155/2018/3506874 DO - 10.1155/2018/3506874 JF - Analytical Cellular Pathology PB - Hindawi KW - ER -