TY - JOUR AU - Rossi Spadafora, Marcello Salvatore AU - Céspedes, Ghislaine AU - Romero, Sandra AU - Fuentes, Isabel AU - Boada-Sucre, Alpidio A. AU - Cañavate, Carmen AU - Flores-Chávez, María PY - 2014 DA - 2014/12/29 TI -Trypanosoma cruziNecrotizing Meningoencephalitis in a Venezuelan HIV⁺-AIDS Patient: Pathological Diagnosis Confirmed by PCR Using Formalin-Fixed- and Paraffin-Embedded-Tissues SP - 124795 VL - 2014 AB - Coinfections with human immunodeficiency virus (HIV) and infectious agents have been recognized since the early 90s. In the central nervous system (CNS) of HIV⁺patients, parasitic protozoans like Toxoplasma gondiihave been described as responsible for the space occupying lesions (SOL) developed. However, the involvement of Trypanosoma cruziis also described but appears to be less frequent in acquired immunodeficiency syndrome (AIDS) and transplant recipients, associated with necrotizing myocarditis and neurological symptoms related to the occurrence of necrotizing pseudotumoral encephalitis (NPE) and meningoencephalitis (NME). The present work aims to present a Venezuelan case of NME associated with the coinfection of HIV and a T. cruzi-like trypanosomatid as well as its evolution and diagnosis by histopathological techniques, electron microscopy, and PCR methods using formalin-fixed- (FF-) and paraffin-embedded- (PE-) tissues. Postmortem cytological studies of leptomeninges imprints reveal the presence of trypomastigotes of Trypanosomasp. Histopathological and electron microscopy studies allowed us to identify an amastigote stage and to reject the involvement of other opportunistic microorganisms as the etiological agent of the SOL. The definitive confirmation of T. cruzi随着病因代理人通过PCR suggesting that the NME by T. cruziwas due to a reactivation of Chagas’ disease. SN - 2210-7177 UR - https://doi.org/10.1155/2014/124795 DO - 10.1155/2014/124795 JF - Analytical Cellular Pathology PB - Hindawi Publishing Corporation KW - ER -